Handbook of Biological Confocal Microscopy

James B. Pawley

1016 pages, parution le 01/09/2006 (3^eme édition)

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Résumé

This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging.

Sommaire

Foundations of Confocal Scanned Imaging in Light Microscopy
Fundamental Limits in Confocal Microscopy
Quantitative Fluorescence Confocal Laser Scanning Microscopy (CLSM) The Pixilated Image
Laser Sources for Confocal Microscopy
Nonlaser Light Sources: Not Using Laser Light for Confocal Microscopy and How to Use Laser Light When It's All You Have
Objective Lenses for Confocal Microscopy
The Specimen Illumination Path and Its Effect on Image Quality
The Intermediate Optical System of Laserscanning Confocal Microscopes
Intermediate Optics in Nipkow Disk Microscopes
The Role of the Pinhole in Confocal Imaging System Photon Detectors for Confocal Microscopy
The Collection, Processing, and Display of Digital Threedimensional Images of Biological Specimens
Visualization Systems for Multidimensional CLSM Images
Mapping and Measuring Surfaces Using Relection Confocal Microscopy
22 additional articles
Index

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Caractéristiques techniques

	PAPIER
Éditeur(s)	Springer
Auteur(s)	James B. Pawley
Parution	01/09/2006
Édition	3^eme édition
Nb. de pages	1016
Format	22 x 29
Couverture	Relié
Poids	3746g
EAN13	9780387259215
ISBN13	978-0-3872-5921-5