Handbook of Single Molecule Fluorescence Spectroscopy

Chris Gell, David Brockwell, Alastair Smith

262 pages, parution le 17/08/2006

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Résumé

Hands-on, practical introduction to single molecule studies
Topical subject area of rapidly growing importance
Only introductory text to date providing methods and instrumentation
Relates the practical aspects to applications in biophysics, the main area of interest at the moment
Written in a pedagogical style with the newcomer to the field in mind

Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.

Readership: Primary: graduate and postgraduate students in physics, biophysics, biochemistry and structural molecular biology. Secondary: professional scientists in the pharmaceutical industry, postgraduate scientists working in nanotechnology.

Sommaire

Introduction
Single Molecule Fluorescence Techniques
Single Molecule Fluorescence Instrumentation
Preparation of samples for single molecule fluorescence spectroscopy
Fluorescence spectroscopy of freely diffusing single molecules: examples
Fluorescence spectroscopy of immobilised single molecules: examples
The outlook for single molecule fluorescence measurements

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	PAPIER
Éditeur(s)	Oxford University Press
Auteur(s)	Chris Gell, David Brockwell, Alastair Smith
Parution	17/08/2006
Nb. de pages	262
Format	17,5 x 25,5
Couverture	Relié
Poids	714g
Intérieur	Noir et Blanc
EAN13	9780198529422
ISBN13	978-0-19-852942-2