Transgenic Animal Technology

A Laboratory Handbook

Carl A. Pinkert

632 pages, parution le 12/12/2002 (2^eme édition)

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Résumé

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems.

Contents

I- Overview

Introduction to Transgenic Animals Technology

II- Transgenic Animal Production Focusing on the Mouse Model

DNA Microinjection and Transgenic Animal Production
Factors Affecting Transgenic Animal Production
Gene Targeting in Embryonic Stem Cells I: History and Methodology
Gene Targeting in Embryonic Stem Cells II. Conditional Technologies
Retrovirus-Mediated Gene Transfer
Nuclear Transfer Technologies

III- Production of Transgenic Laboratory and Domestic Animal Species

Production of Transgenic Rats
Production of Transgenic Rabbits
Production of Transgenic Fish
Production of Transgenic Poultry
Production of Transgenic Swine by DNA Microinjection
Production of Transgenic Ruminants by DNA Microinjection
Production of Transgenic Nonhuman Primates
Nuclear Transfer Technology in Cattle, Sheep, and Swine

IV- Molecular Biology, Analyses and Enabling Technologies

Vector Design for Transgene Expression
Analysis of Transgene Integration
PCR Optimization for Detection of Transgenic Integration
Analysis of Transgene Expression
Assisted Reproductive Technologies and Embryo Culture Methods for Farm Animals
Databases, Internet Resources and Genetic Nomenclature

L'auteur - Carl A. Pinkert

Dr. Pinkert is an Associate Professor in the Department of Comparative Medicine and Director of the Transgenic Animal/ES Cell Resource at the University of Alabama at Birmingham. He earned his B.S. from Colorado State University, M.S. from Southern Illinois, and Ph.D. in 1983 from the University of Georgia, followed by postdoctoral training at the University of Pennsylvania. He was an Assistant Professor at the University of Missouri and then worked at DNX, Inc. (where his proposal for the National Transgenic Development Facility for the National Institute of Child Health and Human Development was awarded and implemented) before joining the UAB faculty. Dr. Pinkert's primary research efforts involve gene transfer, expression, and regulation using transgenic animal modeling. His work has illustrated the potential of modifying the immune system, enhancing growth performance, and the feasibility of molecular farming. He has published extensively on transgenic modeling, reproductive biology, and embryology of laboratory and domestic animals. He serves as an Editro of Transgenic Research and has served as an ad hoc reviewer or consultant for several journals, government agencies, and companies.

Caractéristiques techniques

	PAPIER
Éditeur(s)	Academic Press
Auteur(s)	Carl A. Pinkert
Parution	12/12/2002
Édition	2^eme édition
Nb. de pages	632
Format	20,5 x 23,5
Couverture	Spirale
Poids	1076g
Intérieur	Noir et Blanc
EAN13	9780125571661
ISBN13	978-0-12-557166-1