Chemical biology

A pratical course

Herbert Waldmann, Petra Janning

208 pages, parution le 30/07/2004

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Résumé

The present selection of 12 inspiring experiments has been carefully selected to cover the most commonly used laboratory techniques for the synthesis and analysis of bioactive compounds. Together, they provide a solid introduction to the skills and techniques that are required for the successful study of Chemical Biology..

Here you will learn how to

synthesize a peptide by solid phase synthesis
modify a protein by covalent attachment of a lipid chain
modify an oligonucleotide and use it for DNA-directed immobilization
isolate an enzyme for in vitro synthesis of a polysaccharide
design a ligand for a protein by molecular modeling
perform a proteome analysis in yeast

All experiments haven been thoroughly tested in the authors' laboratory and come with an easy to follow step-by-step instruction, complemented by abundant practical hints and by a thorough treatment of the underlying theory and mechanisms.

Sommaire

DNA-Synthesis and DNA-Hybridization
Doubly Labeled Peptide Nucleic Acids as Probes for the Detection of DNA Point
Mutations
Synthesis and Characterization of a Covalent Oligonucleotide-Streptavidin
Conjugate for DNA-directed Immobilization
Solid Phase Synthesis of Peptides: Bradykinin Analogs and the Evaluation of Calcium Mobilization in PC-12 Cells
In silico Protein Ligand Design
Lipidation of Proteins and Peptides -
Farnesylation of the Ras protein
Insertion of Lipidated Peptides in Model Membranes
Isolation of Potato Phosphorylase and Enzymatic Synthesis of Amylose
Proteome Analysis -
Identification of Proteins Isolated from Yeast - Lectins
Determination of the Sugar Specificity of Jacalin by a Sugar-Lectin Binding Assay
Combinatorial Chemistry -
Ugi-Synthesis, Genetic Algorithm, and Determination of Enzyme Inhibition
Solid-phase Synthesis of an Antibiotic

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Caractéristiques techniques

	PAPIER
Éditeur(s)	Wiley
Auteur(s)	Herbert Waldmann, Petra Janning
Parution	30/07/2004
Nb. de pages	208
Format	17 x 24
Couverture	Broché
Poids	450g
Intérieur	Noir et Blanc
EAN13	9783527307784