Solid State NMR Spectroscopy for Biopolymers

Hazime Saito, Isao Ando, Akira Naito

470 pages, parution le 31/03/2006

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Résumé

When considering the biological significance and industrial and medical applications of biopolymers, it is crucial to know details of their secondary structure, dynamics and assembly. The biopolymers include globular, membrane and fibrous proteins, polypeptides, nucleic acids, polysaccharides and lipids. Solid state NMR spectroscopy has proved to be the most suitable and unrivaled means for investigations of biopolymers. The major advantage of solid state NMR spectroscopy is that the resulting line widths can be manipulated experimentally and are not influenced by motional fluctuation of proteins under consideration as a whole.

Solid State NMR Spectroscopy for Biopolymers: Principles and Applications provides a comprehensive account on how the conformation and dynamics of such biopolymers can be revealed by solid state NMR spectroscopy. Special efforts have been made towards the historical and chronological consequences of a variety of applications and the dynamic aspects of the biopolymer system. In particular, the authors emphasise how important it is to record the most simple DD-MAS (one pulse excitation with high power decoupling) as a mean of locating very flexible portions of membrane proteins and membrane associated peptides. The authors also demonstrate that dynamic features of membrane proteins with a timescale of fast (10⁸ Hz) and intermediate (10⁴ -10⁵ Hz) fluctuation motions can be revealed easily by specific suppression of peaks.

Written for: Biophysicists, biochemists, chemists including NMR spectroscopists, structural biologists, polymer scientists among students, faculties and research scientists at pharmaceutical and chemical industries

Sommaire

Solid state NMR approach: CP-MAS and DD-MAS NMR
Brief outline of NMR parameters
Multinuclear approaches
Experimental strategies
NMR constraints for structural determination
Dynamics
Hydrogen bonded systems
Fibrous proteins
Polysaccharides
Globular proteins
Membrane protein I: dynamic picture
Membrane proteins II: 3D structure
Biologically active membrane-associated peptides
Amyloid and related biomolecules

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Caractéristiques techniques

	PAPIER
Éditeur(s)	Springer
Auteur(s)	Hazime Saito, Isao Ando, Akira Naito
Parution	31/03/2006
Nb. de pages	470
Format	16 x 24
Couverture	Relié
Poids	885g
Intérieur	Noir et Blanc
EAN13	9781402043024
ISBN13	978-1-4020-4302-4