The Condensed Protocols

From Molecular Cloning - A Laboratory Manual

Joseph Sambrook, David William Russell

808 pages, parution le 01/05/2006

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Résumé

The Condensed Protocols From Molecular Cloning: A Laboratory Manual is a single-volume adaptation of the three-volume third edition of Molecular Cloning: A Laboratory Manual. This condensed book contains only the step-by-step portions of the protocols, accompanied by selected appendices from the world's best-selling manual of molecular biology techniques. Each protocol is cross-referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.

Sommaire

Using Plasmid Vectors in Molecular Cloning
Bacteriophage ? and Its Vectors
Working with Bacteriophage M13 Vectors
Working with High-capacity Vectors
Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis
Preparation and Analysis of Eukaryotic Genomic DNA
Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
In Vitro Amplification of DNA by the Polymerase Chain Reaction
Preparation of Radiolabeled DNA and RNA Probes
Working with Synthetic Oligonucleotide Probes
Preparation of cDNA Libraries and Gene Identification
DNA Sequencing
Mutagenesis
Screening Expression Libraries
Expression of Cloned Genes in Escherichia coli
Introducing Cloned Genes into Cultured Mammalian Cells
Analysis of Gene Expression in Mammalian Cells
Protein Interaction Technologies
Appendices
Index

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Caractéristiques techniques

	PAPIER
Éditeur(s)	Cold Spring Harbor Laboratory Press
Auteur(s)	Joseph Sambrook, David William Russell
Parution	01/05/2006
Nb. de pages	808
Format	21 x 28
Couverture	Broché
Poids	2029g
Intérieur	Noir et Blanc
EAN13	9780879697716
ISBN13	978-0-879697-71-6