Fluorescent and Luminescent Probes

A Practical Guide to Technology for Quantitative Real-Time Analysis

W. T. Mason, Bill T. Mason - Collection Biological Techniques Series

648 pages, parution le 16/04/1999 (2^eme édition)

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Résumé

The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.

The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research.

Audience: Cell biologists, biochemists, molecular biologists, and physiologists; postgraduates and research workers in both academia and industry and clinical researchers. This book will be invaluable for those wishing to start using optical probes and also for researchers in the field who want to use the most up-to-date techniques.

Sommaire

Introduction to Fluorescence Microscopy
Optical Probes and Their Applications
Using Optical Probes in Cells - Practicalities, Problems, and Pitfalls
Optical Probes for Specific Molecules, Organelles, and Cells:
Technology for Qulaitative and Quantitative Detection of Optical Probes in Living Cells
Using Novel Indications for Genetic, Molecular, and Cellular Function
Applications of Confocal Microscopy for Optical Probe Imaging
Advanced Imaging and Light Detection Approaches for Optical Probe Applications
CCD Cameras: Key Enabling Technologies for Optical Probe Imaging
Flow Cytomeric Methodologies for Measurement of Optical Probes in Living Cells
Applications of Optical Probe Imaging to Biological Problems
Index

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Caractéristiques techniques

	PAPIER
Éditeur(s)	Elsevier
Auteur(s)	W. T. Mason, Bill T. Mason
Collection	Biological Techniques Series
Parution	16/04/1999
Édition	2^eme édition
Nb. de pages	648
Format	22 x 25
Couverture	Spirale
Poids	1606g
EAN13	9780124478367
ISBN13	978-0-12447-836-7