Molecular Cloning - Set of Volumes 1, 2 and 3

A Laboratory Manual

Joseph Sambrook, David William Russell

2368 pages, parution le 05/12/2000 (3^eme édition)

Ajouter à une liste

Indisponible

Résumé

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity.

In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.

Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today's biomolecular techniques.

The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.

These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing.

The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions.

The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.

As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

Sommaire

Plasmids and Their Usefulness in Molecular Cloning
Bacteriophage lambda and Its Vectors
Working with Bacteriophage M13 Vectors
Working with High-capacity Vectors
Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis
Preparation and Analysis of Eukaryotic Genomic DNA
Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
In vitro Amplification of DNA by the Polymerase Chain Reaction
Preparation of Radiolabeled DNA and RNA Probes
Working with Synthetic Oligonucleotide Probes
Preparation of cDNA Libraries and Gene Identification
DNA Sequencing
Mutagenesis
Screening Expression Libraries
Expression of Cloned Genes in Escherichia coli
Introducing Cloned Genes into Cultured Mammalian Cells
Analysis of Gene Expression in Cultured Mammalian Cells
Protein Interaction Technologies
Appendices

Voir tout

Replier

Caractéristiques techniques

	PAPIER
Éditeur(s)	Cold Spring Harbor Laboratory Press
Auteur(s)	Joseph Sambrook, David William Russell
Parution	05/12/2000
Édition	3^eme édition
Nb. de pages	2368
Format	9 x 14
Couverture	Relié
Poids	13500g
EAN13	9780879695774
ISBN13	978-0-879695-77-4